Salacia chinensis- inhibition of cancer cell

Source : www.vienduoclieu.org.vn/ I/ Salacia chinensis : - Scientific name: Salacia chinensis, a species of vines, 1-2 m tall, twigs, leaves green and brown underneath, berries, same pear, red fruit tip. II/ Medicine : - This is the kind of specific drugs, potentially limiting the growth of cancer cells. - Salacia chinensis is being studied by many scientists. In Vietnam, in addition to Bach Ma National Park, conservation of genetic resources is Salacia chinensis. - At Mountain Kim Phung, Hai Cat village, Huong Tho commune, Huong Tra district, Thua Thien Hue Province, people have discovered the medicinal species. - Form Salacia chinensis plants obtained in  Loc Tri commune, Phu Loc district, Thua Thien Hue. - The team shall classify specimens and dried at 300C and minced. - Dry powder is soaked twigs Salacia chinensis methanol extraction (80%) 3 times at room temperature. - The methanol extract, solvents, then add water and extract the distribution of n-hexane, respectively, ethyl acetate and n-butaol. The extracts were dried and distilled solvent to obtain the corresponding residue n-hexane extract (6.81 g), ethyl acetate (7.4 g), n-butaol (11.5 g). - The results of the study showed that, by special methods, combined with modern spectroscopic methods, have been isolated and identified 3 tritecpen of n-hexane extract of twigs Salacia chinensis in Vietnam, signed effective as S2, S3, S4. - Quality S2 is needle-shaped crystals, white, melting point of 272-2730C. - IR spectrum for the characteristic absorption peak of the ketone group at 1701 cm-1 and no absorption peak of the double bond. - EI-MS spectrum of this substance for molecular ion peak at m / z 426 M +1. 13C-NMR spectrum showed S2 molecules with carbon 29. Molecular formula was deduced from the DEPT spectrum and mass spectrometry data is C29H46O2. - With S4, 13C-NMR and 1H spectrum showed a 6 level 3 in the methyl group from 0.76 to 1.07 ppm. - EI-MS mass spectrum for the peak at m / z 425 [M + H-H2O], corresponding to the molecular formula is C30H50O2. - Quality S3 needles, EI-MS mass spectrum for molecular ion peak at m / z 442 corresponding to the molecular formula is C30H50O2. 1H and 13C-NMR spectrum showed a tritecpen S3, the S2 group similar functions, including 6 grade 3 methyl groups, one group isopropeny. - When comparing the chemical shifts of the methyl group of rank 3 of 6 of S3, S2 and S4 with nature, the authors say, the quality is S3khong hopan frame. - The secondary data shows that the S3 is 20 (29)-lupen-3, 28 - dinol, called betulin. This is a tritecpen present in many plant species belonging to different families. - Belutin showing cytotoxic activity on 2 lines Hela and Hep-2 with the IC 50 values ​​of 40 microg / ml. It also shows anti-HIV activity with IC 50 values ​​of 6.1 microg / ml. This is the first 3 frames tritecpen Lupan hopan and isolated from this plant.